Cross Ovulation Test

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The Blue Cross One Step Urine Home Screening Ovulation Test is a rapid chromatographic immunoassay for the qualitative detection of luteinizing hormone (LH) in urine to aid in the detection of ovulation.



Ovulation is the release of an egg from the ovary. The egg then passes into the fallopian tube where it is ready to be fertilized. In order for pregnancy to occur, the egg must be fertilized by sperm within 24 hours after its release. Immediately prior to ovulation, the body produces a large amount of luteinizing hormone (LH) which triggers the release of a ripened egg from the ovary. This “LH surge” usually takes place in the middle of the menstrual cycle.

The Blue Cross One Step Urine Home Screening Ovulation Test is a complete system to help you predict the time of ovulation, and peak fertility. It is during this fertile time that pregnancy is most likely to occur.

The Blue Cross One Step Urine Home Screening Ovulation Test detects the LH surge in urine, signaling that ovulation is likely to occur in the next 24-36 hours.

Important: The LH surge and ovulation may not occur in all cycles.

Directions for Use:

Allow the test device, urine specimen and/or controls to equilibrate to room temperature (15-30°C) prior to testing.

  1. Determine the day you will begin testing.
  2. Bring the pouch to room temperature before opening it. Remove the test device from the sealed pouch and use it as soon as possible.
  3. Place the test device on a clean and level surface. Hold the dropper vertically and transfer 3 full drops of urine (approx. 100µl) to the specimen well (S) of the test device, and then start the timer. Avoid trapping air bubbles in the specimen well (S).
  4. Wait for the red line(s) to appear. The result should be read at 5 minutes. Do not interpret the result after 10 minutes.


Can I use the Blue Cross One Step Urine Home Screening Ovulation Test Device to avoid pregnancy?
NO. The test should not be used as a form of birth control.  
Do alcohol or common medications affect the test?
NO. But you should consult your physician if you are taking any  hormonal medication. Also, recent oral contraceptive use, breastfeeding or pregnancy could affect the results.  
How long will the line remain visible?
The test should be read at 5 minutes for best results. A positive (surge) result will never disappear. The colored line(s) may become darker and a tinted background may appear after several hours. Some negative results may later display a faint second color line because of evaporation from the test region, which prevents complete migration of the test chemicals. Therefore, you should not read the result after 10 minutes and discard the test strip once you have read the test.  
Once I see a positive result, when is the best time to have intercourse?
Ovulation is likely to occur within 24-36 hours. This is your most fertile time. Sexual intercourse within this time frame is advised.  
I am now using the basal body temperature method (BBT). Does this test replace BBT?
The shift in basal body temperature primarily indicates that ovulation has already occurred. The Blue Cross One Step Urine Home Screening Ovulation Test device (urine) indicates that ovulation is about to occur.  
I have received a positive result and had intercourse during these fertile days. I have not become pregnant. What shall I do?
There are many factors that can affect your ability to become pregnant. Often you may need to use the test kit for 3 - 4 months before achieving pregnancy. You and your partner should consult your physician if pregnancy is not achieved after 3 - 4 months.  
I have had a positive result and had intercourse during these fertile days. I think I may be pregnant. How soon can I find out?
The pregnancy products can provide you with results as early as the first day you missed your period.  
What time of the day should I perform the test? Do I need to use first morning urine?
We do not recommend first morning urine, but any other time of day is suitable. For best results, try to collect your urine at approximately the same time each day.  
Will the amount of liquid I drink affect the result?
Heavy intake of fluids prior to testing will dilute the hormone in your urine. We suggest that you limit your fluid intake for about two hours before you collect your urine.


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